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Your Position: 홈 > Protein > LAG-3 > LA3-H52H3

Human LAG-3 / CD223 Protein, His Tag (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    LAG3,CD223,FDC
  • Source
    Human LAG-3 / CD223 Protein, His Tag(LA3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
    Request for sequence
  • Molecular Characterization
    LAG-3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 48.1 kDa. The protein migrates as 58-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LAG-3 SDS-PAGE

Human LAG-3 / CD223 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
LAG-3 MALS images

The purity of Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) is more than 90% and the molecular weight of this protein is around 60-85 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
LAG-3 ELISA

Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

Protocol
LAG-3 ELISA

Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG4 (S108P) with a linear range of 0.2-4 ng/mL (Routinely tested).

Protocol
LAG-3 ELISA

Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-LAG3 Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (Routinely tested).

Protocol
LAG-3 ELISA

Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 5 μg/mL, add Anti-PD1 crossFab x LAG3, Human IgG1 and then add Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) at 0.2 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).

Protocol
  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • Clinical and Translational Updates

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