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Your Position: > Kits > FMC63 > RAB-P001

Anti-CD19 (FMC63) CAR Immunogenicity ELISA Kit

For research use only.
    Materials Provided
    IDComponentsSize
    RAB001-C01High-bind Plate1 plate
    RAB001-C02Mouse FMC63 scFv25 μg
    RAB001-C03FMC63 ADA Standard15 μg
    RAB001-C04Biotin-Mouse FMC63 scFv20 μg
    RAB001-C05Streptavidin-HRP50 μL
    RAB001-C06Coating Buffer12 mL
    RAB001-C0710xWashing Buffer 50 mL
    RAB001-C08Blocking Buffer50 mL
    RAB001-C09Substrate Solution12 mL
    RAB001-C10Stop Solution7 mL
  • Product Overview
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.Anti-fmc63 scFv antibody can specifically bind to the antigen recognition epitope of fmc63 scFv on anti-CD19 car, and it shows high specificity and sensitivity,it's used to detect the expression of fmc63 scFv derived car.
  • Application

    This kit is developed for detection of Anti-CD19 (FMC63) antibodies in CAR Immunogenicity assay test. Detection and monitoring the level of ADA is important to fully understand the potential immune responses.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
  • Assay Principles
    This assay kit employs a bridging-ELISA format, providing a rapid detection of FMC63 ADA. The kit consists High-bind Plate and Mouse FMC63 scFv and FMC63 ADA Standard and Biotin-Mouse FMC63 scFv and Streptavidin-HRP and buffers.

    Your experiment will include 4 simple steps:

    a) Coat the plate with Mouse FMC63 scFv.

    b) Add your molecule of interest to the tests.

    c) Add Mouse FMC63 scFv-Biotin.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Typical Data Please refer to DS document for the assay protocol.
 FMC63 TYPICAL DATA

Detection of FMC63 ADA titer by bridging-ELISA Assay.
Immobilized Mouse FMC63 scFv can bind FMC63 ADA in 1:20 human serum, and then add Biotin-Mouse FMC63 scFv. Detection was performed using HRP-conjugated streptavidin with sensitivity of 19 ng/mL (QC tested).

  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(USD) : 1235.00

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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