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Your Position: 홈 > Protein > LAG-3 > LA3-M82E5

Biotinylated Mouse LAG-3 / CD223 Protein, His,Avitag™ (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    LAG3,CD223,FDC
  • Source
    Biotinylated Mouse LAG-3, His,Avitag(LA3-M82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Leu 442 (Accession # Q61790-1).
    Predicted N-terminus: Gly 24
    Request for sequence
  • Molecular Characterization
    LAG-3 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 48.8 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LAG-3 SDS-PAGE

Biotinylated Mouse LAG-3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
LAG-3 MALS images

The purity of Biotinylated Mouse LAG-3, His,Avitag (Cat. No. LA3-M82E5) is more than 90% and the molecular weight of this protein is around 112-136 kDa verified by SEC-MALS.

Report
  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • Clinical and Translational Updates

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