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Your Position: > Protein > NT-3 > NT3-H5213

Human NT-3 Protein, Tag Free (HPLC verified)

  • Synonym
    HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,NTF3,NT3.Neurotrophin3
  • Source
    Human NT-3 Protein, Tag Free(NT3-H5213) is expressed from human 293 cells (HEK293). It contains AA Tyr 139 - Thr 257 (Accession # P20783).
    Predicted N-terminus: Tyr 139
  • Molecular Characterization
    NT-3 Structure

    This protein carries no "tag".

    The protein has a calculated MW of 13.6 kDa. The protein migrates as 13-14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
NT-3 SDS-PAGE

Human NT-3 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
NT-3 SEC-HPLC

The purity of Human NT-3 Protein, Tag Free (Cat. No. NT3-H5213) was greater than 95% as determined by SEC-HPLC.

Bioactivity-ELISA
 NT-3 ELISA

Immobilized Human NT-3 Protein, Tag Free (Cat. No. NT3-H5213) at 1 μg/mL (100 μL/well) can bind Human TrkC Protein, Fc Tag (Cat. No. TRC-H5256) with a linear range of 0.8-13 ng/mL (QC tested).

  • Background
    Neurotrophin-3 (NT-3), encoded by the NTF3 gene, is a member of the NGF family of neurotrophic factors (also named neurotrophins). The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytoki.
  • Clinical and Translational Updates

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Datasheet & Documentation

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DMF (Drug Master File)

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