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Your Position: > Protein > SIRP alpha > SIA-C82F3

Biotinylated Cynomolgus SIRP alpha / CD172a Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
  • Source
    Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag(SIA-C82F3) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 370 (Accession # NP_001271679.1).
    Predicted N-terminus: Glu 31
  • Molecular Characterization
    SIRP alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 65.3 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SIRP alpha SDS-PAGE

Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
SIRP alpha MALS images

The purity of Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-C82F3) is more than 90% and the molecular weight of this protein is around 160-190 kDa verified by SEC-MALS.

Bioactivity-ELISA
 SIRP alpha ELISA

Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag (Cat. No. CD7-C5252) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-C82F3) with a linear range of 1-78 ng/mL (QC tested).

 SIRP alpha ELISA

Immobilized Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-C82F3) with a linear range of 0.005-0.313 μg/mL (Routinely tested).

  • Background
    Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • Clinical and Translational Updates

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