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Your Position: 홈 > Protein > CD47 > CD7-M82F5

Biotinylated Mouse CD47 Protein, Fc,Avitag™ (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Biotinylated Mouse CD47 Protein, Fc,Avitag(CD7-M82F5) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Lys 140 (Accession # Q61735-1).
    Predicted N-terminus: Gln 19
    Request for sequence
  • Molecular Characterization
    CD47 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 42.0 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD47 SDS-PAGE

Biotinylated Mouse CD47 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
CD47 MALS images

The purity of Biotinylated Mouse CD47 Protein, Fc,Avitag (Cat. No. CD7-M82F5) is more than 80% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
CD47 ELISA

Immobilized Biotinylated Mouse CD47 Protein, Fc,Avitag (Cat. No. CD7-M82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse SIRP alpha Protein, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) with a linear range of 0.01-1.25 μg/mL (QC tested).

Protocol
  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • Clinical and Translational Updates

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