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Your Position: > Protein > LILRB4 > LI4-H82F6

Biotinylated Human LILRB4 / CD85k / ILT3 Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    LILRB4,ILT3,LIR5,CD85K,HM18
  • Source
    Biotinylated Human LILRB4 Protein, Fc,Avitag(LI4-H82F6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    LILRB4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 54.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LILRB4 SDS-PAGE

Biotinylated Human LILRB4 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
LILRB4 MALS images

The purity of Biotinylated Human LILRB4 Protein, Fc,Avitag (Cat. No. LI4-H82F6) is more than 90% and the molecular weight of this protein is around 110-130 kDa verified by SEC-MALS.

Bioactivity-ELISA
 LILRB4 ELISA

Immobilized Anti-LILRB4 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human LILRB4 Protein, Fc,Avitag (Cat. No. LI4-H82F6) with a linear range of 0.1-4 ng/mL (QC tested).

  • Background
    Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • Clinical and Translational Updates

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