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Your Position: 홈 > Protein > ADORA2A > ADA-H52P4

Human ADORA2A Full Length Protein (VLP)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    ADORA2A,adenosine A2a receptor,A2aR, ADORA2, RDC8
  • Source
    Human ADORA2A Full Length Protein-VLP(ADA-H52P4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ser 412 (Accession # P29274-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization

    The protein has a calculated MW of 47.9 kDa | 56.1 kDa.

    Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

    The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
ADORA2A ELISA

Immobilized Human ADORA2A Full Length Protein-VLP (Cat. No. ADA-H52P4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ADORA2A antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Protocol
Identity-DLS
ADORA2A DLS

The mean peak Radius of VLP is 55-70 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

  • Background
    Adenosine Receptor Subtype A2a (ADORA2A) is a G-protein coupled adenosine receptor encoded by the ADORA2A gene. It is mainly involved in pathophysiological processes including neuroinflammation, synaptic plasticity in glutamatergic synapses and neurogenesis.
  • Clinical and Translational Updates

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