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Your Position: > Protein > Spike protein > SPN-C522a
  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    SARS-CoV-2 Spike Trimer, His Tag (BA.1/Omicron) (SPN-C522a) is expressed from human 293 cells (HEK293). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.1; GISAID clade: GRA). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 138.2 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Spike protein SDS-PAGE

SARS-CoV-2 Spike Trimer, His Tag (BA.1/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike protein MALS images

The purity of SARS-CoV-2 Spike Trimer, His Tag (BA.1/Omicron) (Cat. No. SPN-C522a) is more than 85% and the molecular weight of this protein is around 500-550 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike protein ELISA

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.1/Omicron) (Cat. No. SPN-C522a) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).

 Spike protein ELISA

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.1/Omicron) (Cat. No. SPN-C522a) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Omicron Specific) (Cat. No. SPD-M305) with a linear range of 0.1-4 ng/mL (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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