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Your Position: > Antibody > FMC63 > FM3-BY54

Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag™ (Y45) DMF

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  • Source
    Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag™ (Y45) is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293). Biotinylation of this antibody is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Application
    Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).
  • Clone
    Y45
  • Species
    Mouse
  • Isotype
    Mouse IgG1 & | kappa
  • Specificity
    Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
  • Immunogen

    Recombinant FMC63 scFv derived from HEK293 cells.

  • Conjugate

    Biotin

  • Isotype Control

    The Isotype control is sold separately and you can search for Cat. No. DNP-BM190 for product information.

  • Recommended Dilution
    1:50
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Bioactivity-FACS
 FMC63 FACS

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag (Cat. No. FM3-BY54) and isotype control respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

 FMC63 FACS

Non-specificity of Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag (Cat. No. FM3-BY54) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 502,500

가격(KRW) : 3,118,500

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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