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Your Position: > Protein > BAFFR > BAR-H82E3

Biotinylated Human BAFFR / TNFRSF13C Protein, His,Avitag™ (MALS verified)

  • 동의어
    BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R
  • 출처
    Biotinylated Human BAFFR, His,Avitag (BAR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).
    Predicted N-terminus: Ser 7
  • 분자 표징
    Online(Ser 7 - Ala 71) Q96RJ3-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 10.1 kDa. The protein migrates as 15-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) SDS-PAGE gel

Biotinylated Human BAFFR, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human BAFFR, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) is more than 85% and the molecular weight of this protein is around 12-20 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human BAFFR, His,AvitagBiotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) ELISA bioactivity

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 1 μg/mL (100 μL/well) can bind Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) with a linear range of 0.2-2 ng/mL (QC tested).

Biotinylated Human BAFFR, His,AvitagBiotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) ELISA bioactivity

Immobilized Anti-BAFFR Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) with a linear range of 0.2-0.8 ng/mL (Routinely tested).

Biotinylated Human BAFFR, His,AvitagBiotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) ELISA bioactivity

Immobilized Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).

  • 백그라운드
    BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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