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Your Position: > Protein > B7-H5 > B75-H5258

Human B7-H5 / Gi24 / VISTA Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    B7-H5,SISP1,Gi24,VISTA
  • Source
    Human B7-H5 Protein, Mouse IgG2a Fc Tag(B75-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568.1).
    Predicted N-terminus: Phe 33
  • Molecular Characterization
    B7-H5 Structure

    This protein carries a mouse IgG2a Fc tag at the C-terminus

    The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-H5 SDS-PAGE

Human B7-H5 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 B7-H5 ELISA

Immobilized Biotinylated Human VSIG3, Fc,Avitag (Cat. No. VS3-H82F9) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-H5 Protein, Mouse IgG2a Fc Tag (Cat. No. B75-H5258) with a linear range of 0.039-0.625 μg/mL (QC tested).

Bioactivity-SPR
 B7-H5 SPR

Human B7-H5 Protein, Mouse IgG2a Fc Tag (Cat. No. B75-H5258) Captured on CM5 chip via anti-mouse antibodies surface can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 8.87 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 B7-H5 BLI

Loaded Biotinylated Human VSIG3, Fc,Avitag (Cat. No. VS3-H82F9) on SA Biosensor, can bind Human B7-H5, Mouse IgG2a Fc Tag (Cat. No. B75-H5258) with an affinity constant of 2.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:6 Details
  • Latest Research Phase:Phase 2 Clinical

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DMF (Drug Master File)

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