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Your Position: 홈 > Protein > Fc gamma RIIIB / CD16b (NA1) > CDB-H82E4

Biotinylated Human Fc gamma RIIIB / CD16b (NA1) Protein, His,Avitag™ (SPR & BLI & MALS verified)

소개
QC & Assay Protocol
Citation & 백그라운드
  • 동의어
    Fc gamma RIIIB , CD16b (NA1),FCGR3B,CD16B,FCG3B,FCGR3,FCG3,IGFR3
  • 출처
    Biotinylated Human CD16b (NA1), His,Avitag (CDB-H82E4) is expressed from human HEK293 cells. It contains AA Gly 17 - Ser 200 (Accession # AAA35881.1). The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.
    Predicted N-terminus: Gly 17
    Request for sequence
  • 분자 표징
    Online(Gly 17 - Ser 200) AAA35881.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 24.6 kDa. The protein migrates as 38-48 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) SDS-PAGE gel

Biotinylated Human CD16b (NA1), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) is more than 90% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.

Report
Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 8.32 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) on SA Chip, can bind Herceptin with an affinity constant of 3.31 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol
Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) on SA Biosensor, can bind Rituximab with an affinity constant of 7.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
  • 백그라운드
    CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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