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Human IL-10 Protein, His Tag

  • Synonym
    CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10
  • Source
    Human IL-10, His Tag(IL0-H4248) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1).
    Predicted N-terminus: His
  • Molecular Characterization
    IL-10 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-10 SDS-PAGE

Human IL-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 IL-10 ELISA

Immobilized Human IL-10, His Tag (Cat. No. IL0-H4248) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc,Avitag (Cat. No. ILR-H82F6) with a linear range of 0.2-10 ng/mL (QC tested).

Bioactivity-Bioactivity CELL BASE
 IL-10 CELL

Human IL-10, His Tag (Cat. No. IL0-H4248) inhibits LPS-induced secretion of IL-6 by RAW264.7 cells. The EC50 for this effect is 0.11-0.24 ng/mL (Routinely tested).

  • Background
    Interleukin-10 (IL-10) is also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Mature human IL-10 shares 72% - 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. Knockout studies suggested the function of Interleukin-10 / IL-10 as an essential immunoregulator in the intestinal tract. Patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin-10, showing the importance of interleukin-10 for counteracting excessive immunity in the human body.
  • Clinical and Translational Updates

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