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His

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HIS-PFY63-Cell-based assay
 His FACS

Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with 100 μL of 10 μg/mL Human CD19 (20-291) Protein, His Tag (Cat. No. CD9-H52H2) and negative control protein respectively, washed and then followed with PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) (Cat. No. HIS-PFY63) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

HIS-M535-SPR
 His SPR

Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) captured on Protein G Chip can bind Rabbit Serum Albumin, His Tag (Cat. No. RSA-R52H6) with an affinity constant of 29.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

HIS-M535-ELISA
 His ELISA

Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody, Human IgG1(AY63) (Cat. No. HIS-M535) with a linear range of 0.1-1 ng/mL (QC tested).

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