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Your Position: > Antibody > His > HIS-FM535

Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (MALS verified)

  • Source

    Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    55F8

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Acridine ester

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Tag

  • Immunogen

    A protein peptide with his tag.

  • Specificity

    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
His SDS-PAGE

Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
His MALS images

The purity of Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is more than 85% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Bioactivity-MPCLIA
 His MPCLIA

Immobilized 0.025 μg /Test of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) to the Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) (Cat. No. SCT-M369) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

 His MPCLIA

The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable at 37°C for 48 hours.

 His MPCLIA

The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable after freezing and thawing 3 times.

  • Background
    His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 54,000

가격(KRW) : 499,500

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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