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Your Position: 홈 > Immunohistochemistry (IHC) Antibody

Immunohistochemistry (IHC) Antibody

Immunohistochemistry (IHC) Antibody

Background
Product Features
Target Overview of IHC Antibody Products
Product List
Validation Data
Background
Immunohistochemistry (IHC) is an antibody-based technique that utilizes the specific binding between antigens and antibodies. Through enzyme-labeled antibodies and chromogenic substrate reactions, it enables the localization, qualitative, and quantitative analysis of antigens within tissue cells. Due to its high specificity, sensitivity, and precise localization, IHC effectively integrates morphological and functional studies, making it widely applied across various fields of biological and medical research.
Due to its ability to visually and  qualitatively detect target proteins in tissue samples, IHC has become an essential validation method in the development of solid tumor-targeted therapies such as antibody-drug conjugates (ADCs) and TIL-based cell therapies. To better support drug development efforts, ACROBiosystems, through its ACRODiagnostic pathology platform, has conducted targeted IHC methodological validation for its antibody products, ensuring their specificity and suitability for IHC applications. Validation is performed using both healthy and tumor tissue samples to confirm accurate staining localization and applicability across representative cancer types. Furthermore, to meet diverse needs, our antibody development and pathology platforms offer customized IHC antibody development and screening services, allowing tailored solutions based on specific research requirements.
Product Features

Sufficient Validation

Aligned with cutting-edge biologics research

Antibody development focuses on the top 30 ADC and cell therapy targets, tailored for biomarker detection applications.

Reliable Quality

Validated to meet IHC clinical practices

Covers biological model validation and pathological QC testing to ensure compliance with IHC clinical practice requirements.

Flexible Authorization

Ready-to-use options for easy application

Provides fully validated ready-to-use working solutions, reducing assay development time by up to 20%.

Flexible Authorization

Consistent and stable product availability

Diagnostic-grade materials ensure a reliable supply chain, supporting consistent high-quality production and continuous availability.

Target Overview of IHC Antibody Products
BRAIN & NECK & ESOPHAGUS
LUNG
LIVER
BREAST
PANCREAS
STOMACH
POLOM
PROSTATE
Gland
Others
Product List

  • ALL

  • ADC

  • CGT

  • Clinical

MolecularCat. No.Product DescriptionPreorder/Order
MolecularCat. No.Product DescriptionPreorder/Order
MolecularCat. No.Product DescriptionPreorder/Order
MolecularCat. No.Product DescriptionPreorder/Order
Validation Data & Application Example
Claudin-18.2 IHC Antibody（Cat. No. HCS-S278）
Example of cross-reactivity: According to the Claudin-18.2 immunohistochemical cross-reactivity experiment, there is no cross-reactive staining result between Claudin-18.2 and Claudin-18.1.
> Use Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain normal gastric tissue, strong positive staining of gastric gland cell cytoplasm and/or cell membrane, negative staining of other tissue cells.

Human Stomach Tissue, 4X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10)(HCS-S278)Human Stomach Tissue, 4X

Human Stomach Tissue, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Stomach Tissue, 20X
> Normal lung tissue is stained with Claudin-18.2 primary antibody (Cat. No. HCS-S278), and lung tissue cells are negatively stained (Claudin-18.2 normal lung 20X).
 

Human Lung Tissue, 4X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Lung Tissue, 4X

Human Lung Tissue, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Lung Tissue, 20X

* The human gene Claudin-18 has two protein isoforms, Claudin-18.1 and Claudin-18.2, which differ within the N-terminal 69 amino acids. Claudin-18.1 expression is restricted to the lungs, whereas Claudin-18.2 expression is restricted to the stomach.

Staining example of human tumor tissue samples: use Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain gastric cancer, colorectal cancer，pancreatic cancer, and ovarian cancer tissue samples respectively
> Using Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain normal gastric tissues, positive cell cytoplasm and/or cell membrane specific positive staining (staining intensity ≥ 1), no non-specific staining (staining intensity < 1) , no background staining (staining intensity < 1 ).

Human Gastric Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Gastric Cancer, 20X

Human Colorectal Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10)(HCS-S278)Human Colorectal Cancer, 20X

Human Pancreatic Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Pancreatic Cancer, 20X

Human Ovarian Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Ovarian Cancer, 20X
Staining example of applicable platform: use Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain normal gastric tissues at Leica BOND-III and Dako link 48.

Leica BOND-III-10X

Leica BOND-III-10X

Leica BOND-III-40X

Leica BOND-III-40X

Dako link 48-10X

Dako link 48-10X

Dako link 48-40X

Dako link 48-40X
Performance comparison
Titer: ACRO at 1/1000 dilution and Competitor A at 1/200 dilution, color intensity is similar
> Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin-18.2 with ACRO/Competitor A. Membranous staining on human gastric epithelial cells is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Claudin18.2 (ACRO)
1:1000-40X

Claudin18.2 (ACRO)1:1000-40X

Claudin18.2 (Competitor A)
1:200-40X

Claudin18.2 (Competitor A)1:200-40X

Claudin18.2 (Competitor A)
1:500-40X

Claudin18.2 (Competitor A)1:500-40X
Sensitivity: ACRO staining intensity is stronger than Competitor A
> Immunohistochemical analysis of paraffin-embedded human gastric cancer labeling Claudin-18.2 with ACRO/Competitor A. Membranous staining on tumor cells is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Claudin18.2 (ACRO)
1:1000-4X

Claudin18.2 (ACRO)1:1000-4X

Claudin18.2 (Competitor A)
1:200-4X

Claudin18.2 (Competitor A)1:200-4X

Claudin18.2 (Competitor A)
1:500-4X

Claudin18.2 (Competitor A)1:500-4X

Claudin18.2 (ACRO)
1:1000-40X

Claudin18.2 (ACRO)1:1000-40X

Claudin18.2 (Competitor A)
1:200-40X

Claudin18.2 (Competitor A)1:200-40X

Claudin18.2 (Competitor A)
1:500-40X

Claudin18.2 (Competitor A)1:500-40X
ACRO Quality

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