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Your Position: > Kits > IL-8 > CEA-C004

ClinMax™ Human IL-8 ELISA Kit, PRO

For research use only.
    Product Details
    ClinMax™ Human IL-8 ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-8 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human IL-8 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     IL-8 Workflow
    Key Features
    AnalyteIL-8
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity3.0 pg/mL
    Range6.25 pg/mL-400 pg/mL
    Assay Time1.75 hr
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 uL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 IL-8 TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C004 is less than 3.0 pg/mL.

Validation
Dilution Linearity

Three human serum samples with high concentrations of IL-8 were diluted 1:2, 1:4, 1:8, 1:16, 1:32 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 103.12% of IL-8 was detected from serum samples.

 IL-8 DILUTION LINEARITY
Intra-Assay Statistics

Ten replicates of each of five samples containing different IL-8 concentrations were tested in one assay , Intra-Assay Precision CV<10%.

 IL-8 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of IL-8 were tested in three independent assays , Inter-Assay Precision CV<15%.

 IL-8 INTER-ASSAY STATISTICS
Recovery

Recombinant IL-8 was spiked into 5 human serum samples, and then analyzed. On average, 97.54% of IL-8 was recovered from serum samples.

 IL-8 RECOVERY

Recombinant IL-8 was spiked into cell supernatant sample, and then analyzed. On average, 98.87% of IL-8 was recovered from serum samples.

 IL-8 RECOVERY
Interference effect

Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.

 IL-8 INTERFERENCE EFFECT
Materials Provided
IDComponentsSize
CEA004-C01Pre-coated Anti-IL-8 Antibody Microplate1 plate
CEA004-C02IL-8 Calibrator20 μg×2
CEA004-C03Biotin-Anti-IL-8 Antibody Concentrated Solution100 μL
CEA004-C04Biotin Antibody Dilution Buffer8 mL
CEA004-C05IL-8 Streptavidin-HRP Concentrated Solution0.5 mL
CEA004-C06Streptavidin-HRP Dilution Buffer15 mL
CEA004-C0720× Washing Buffer50 mL
CEA004-C08Sample Dilution Buffer15 mL×2
CEA004-C09Substrate Solution12 mL
CEA004-C10Stop Solution6 mL
  • Clinical and Translational Updates

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