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Your Position: > Protein > TM4SF5 > TM5-H52D3

Human TM4SF5 Protein, Flag,His Tag (Detergent)

  • Source
    Human TM4SF5 Protein, Flag,His Tag(TM5-H52D3) is expressed from human 293 cells (HEK293). It contains AA Cys 2 - His 197 (Accession # O14894).
    Predicted N-terminus: Met
  • Molecular Characterization
    TM4SF5 Structure

    This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 24.4 kDa.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
 TM4SF5 ELISA

Immobilized Human TM4SF5 Protein, Flag,His Tag (Cat. No. TM5-H52D3) at 5 μg/mL (100 μL/well) on a Nickel Coated plate can bind Anti-TM4SF5 Antibody with a linear range of 0.039-2.5 μg/mL (QC tested).

Bioactivity-SPR
 TM4SF5 SPR

Anti-TM4SF5 Antibody captured on Protein A Chip can bind Human TM4SF5 Protein, Flag,His Tag (Cat. No. TM5-H52D3) with an affinity constant of 131 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

  • Background
    The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate
  • Clinical and Translational Updates

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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