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Your Position: > Protein > VSIG4 > VS4-H82E3

Biotinylated Human VSIG4 Protein, His,Avitag™ (MALS verified)

  • Synonym
    VSIG4,CRIg,Z39IG
  • Source
    Biotinylated Human VSIG4 Protein, His,Avitag(VS4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 283 (Accession # Q9Y279).
    Predicted N-terminus: Arg 20
  • Molecular Characterization
    VSIG4 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 32.8 kDa. The protein migrates as 43-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
VSIG4 SDS-PAGE

Biotinylated Human VSIG4 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
VSIG4 MALS images

The purity of Biotinylated Human VSIG4 Protein, His,Avitag (Cat. No. VS4-H82E3) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

  • Background
    V-set and immunoglobulin domain-containing protein 4 (VSIG4) is also known as Protein Z39Ig, is a type I transmembrane glycoprotein. VSIG4 is a B7 family-related protein and an Ig superfamily member. VSIG4 contains two Ig-like (immunoglobulin-like) domains. VSIG4 is abundantly expressed in several fetal tissues. In adult tissues, the highest expression of VSIG4 is in lung and placenta. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. VSIG4 is a phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production.
  • Clinical and Translational Updates

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