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Your Position: > Kits > TSLP > CEA-C074

ClinMax™ Human TSLP ELISA Kit

For research use only.
    Product Details
    ClinMax™ Human TSLP ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TSLP that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M28 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human TSLP in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     TSLP Workflow
    Key Features
    AnalyteTSLP
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity<31.25pg/mL
    Range31.25 pg/mL-2000 pg/mL
    Assay Time1 hr 45 min
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 uL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 TSLP TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of TSLP is less than 31.25 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different TSLP concentrations were tested in one assay. Acceptable criteria: CV<10%.

 TSLP INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of TSLP were tested in independent assays. Acceptable criteria: CV<15%.

 TSLP INTER-ASSAY STATISTICS
Recovery

Recombinant TSLP was spiked into 3 human serum samples, and then analyzed. The average recovery of TSLP for serum samples is 91.5%.

 TSLP RECOVERY
Materials Provided
IDComponentsSize
CEA074-C01Pre-coated Anti-TSLP Antibody Microplate1 plate
CEA074-C02Human TSLP Standard20 ug×2
CEA074-C03Biotin-Anti-TSLP Antibody Con. Solution100 μL
CEA074-C04Biotin-Antibody Dilution Buffer8 mL
CEA074-C05Streptavidin-HRP Con. Solution500 μL
CEA074-C06Streptavidin-HRP Dilution Buffer15 mL
CEA074-C0720× Washing Buffer50 mL
CEA074-C08Sample Dilution Buffer15 mL×2
CEA074-C09Substrate Solution12 mL
CEA074-C10Stop Solution6 mL
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 747,000

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