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ClinMax™ Human ANGPTL3 ELISA Kit, PRO

For research use only.
    Product Details
    ClinMax™ Human ANGPTL3 ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human ANGPTL3 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M16 guideline.
    2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    3. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human ANGPTL3 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     ANGPTL3 Workflow
    Key Features
    AnalyteANGPTL3
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity0.156 ng/mL
    Range0.156 ng/mL-10 ng/mL
    Assay Time2.75 hr
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 uL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 ANGPTL3 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of ANGPTL3 is less than 0.156 ng/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different ANGPTL3 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 ANGPTL3 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of ANGPTL3 were tested in independent assays. Acceptable criteria: CV<15%.

 ANGPTL3 INTER-ASSAY STATISTICS
Recovery

Recombinant ANGPTL3 was spiked into 3 human serum samples, and then analyzed. The average recovery of ANGPTL3 for serum samples is 100.5%.

 ANGPTL3 RECOVERY
Materials Provided
IDComponentsSize
CEA089-B01Pre-coated Anti-ANGPTL3 Antibody Microplate1 plate
CEA089-B02Human ANGPTL3 Standard40 μg×2
CEA089-B03Biotin-Anti-ANGPTL3 Antibody Con. Solution100 μL
CEA089-B04Biotin-Antibody Dilution Buffer8 mL
CEA089-B05Streptavidin-HRP Con. Solution500 μL
CEA089-B06Streptavidin-HRP Dilution Buffer15 mL
CEA089-B0720× Washing Buffer50 mL
CEA089-B082×Sample Dilution Buffer15 mL×2
CEA089-B09Substrate Solution12 mL
CEA089-B10Stop Solution6 mL
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 747,000

가격(KRW) : 3,361,500

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DMF (Drug Master File)

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