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Your Position: > Protein > H-2Kb & B2M & OVA (SIINFEKL) > H2A-M82E6

Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Monomer, MALS verified)

  • Source
    Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein(H2A-M82E6) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide (Accession # P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL).
    Predicted N-terminus: Gly 22 & Ile 21
  • Molecular Characterization

    Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 39.7 kDa and 15.7 kDa. The protein migrates as 48-53 kDa and 17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
H-2Kb & B2M & OVA (SIINFEKL) SDS-PAGE

Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
H-2Kb & B2M & OVA (SIINFEKL) MALS images

The purity of Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Cat. No. H2A-M82E6) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
 H-2Kb & B2M & OVA (SIINFEKL) ELISA

Immobilized Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Cat. No. H2A-M82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind anti-H-2Kb/OVA (SIINFEKL) antibody with a linear range of 0.1-4 ng/mL (QC tested).

 H-2Kb & B2M & OVA (SIINFEKL) ELISA

Immobilized Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Cat. No. H2A-M82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (Routinely tested).

  • Background
    Ovalbumin (OVA) can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. ovalbumin (Ova) was presented to T cells if it was released from pinosomes into the cytosol by osmotic lysis of pinosomes. Immunization with the adjuvanted SIINFEKL peptide induces long-lasting CD8+ T cell immunity in mice.
  • Clinical and Translational Updates

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Datasheet & Documentation

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DMF (Drug Master File)

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