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Your Position: > Protein > PNGase F > PNF-E51H3

PNGase F (500U/ul)

  • Product Details
    PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
  • Application

    Leaves N-glycan core oligosaccharides intact and suitable for further analysis
    Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination

  • Unit Definition
    One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µl.
  • Purity

    >90% as determined by SDS-PAGE.

  • Enzyme Activity
    >500 U/μL
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Notes

    To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.

    PNGase F will not cleave N-linked glycans containing core α1-3 Fucose.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 Mm Tris, 50 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 12 months under sterile conditions.
SDS-PAGE
PNGase F SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity
 PNGase F ENZYME

One unit enzyme to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µl (QC tested).

  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 165,000

가격(KRW) : 660,000

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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