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Your Position: > Protein > B7-H4 > B74-C52H8

Canine B7-H4 Protein, His Tag (MALS verified)

  • Synonym
    B7-H4,VTCN1,B7S1,B7h.5
  • Source
    Canine B7-H4, His Tag(B74-C52H8) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # XP_038547815.1).
    Predicted N-terminus: Phe 29
  • Molecular Characterization
    B7-H4 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.2 kDa. The protein migrates as 44-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-H4 SDS-PAGE

Canine B7-H4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
B7-H4 MALS images

The purity of Canine B7-H4, His Tag (Cat. No. B74-C52H8) is more than 95% and the molecular weight of this protein is around 38-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
 B7-H4 ELISA

Immobilized Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Canine B7-H4, His Tag (Cat. No. B74-C52H8) with a linear range of 1-16 ng/mL (QC tested).

 B7-H4 ELISA

Immobilized Canine B7-H4, His Tag (Cat. No. B74-C52H8) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (Routinely tested).

Bioactivity-SPR
 B7-H4 SPR

Anti-B7-H4 antibody, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Canine B7-H4, His Tag (Cat. No. B74-C52H8) with an affinity constant of 8.59 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
  • Clinical and Translational Updates

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