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Your Position: > Protein > Nucleocapsid protein > NUN-V5145

HCoV-OC43 Nucleocapsid protein, His Tag

  • Synonym
    Nucleocapsid protein,NP,Protein N
  • Source
    HCoV-OC43 Nucleocapsid, His Tag(NUN-V5145) is expressed from E. coli cells. It contains AA Met 1 - Ile 448 (Accession # P33469-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Nucleocapsid protein Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 50.3 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 40 mM PB, 500 mM NaCl, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Nucleocapsid protein SDS-PAGE

HCoV-OC43 Nucleocapsid, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Clinical and Translational Updates

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